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Contrary to oligo(dT) primers, these primers are not template precise and therefore are suitable for DNA synthesis working with Klenow fragments with DNA templates or for cDNA synthesis employing reverse transcriptase with mRNA templates.
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Not like oligo(dT) primers, these primers are certainly not template certain and are well suited for DNA synthesis working with Klenow fragments with DNA templates or for cDNA synthesis making use of reverse transcriptase with mRNA templates.
2. If a repeat databases from the identical organism will not be available, the database in the closest dad or mum of that organism in the taxonomy tree will likely be chosen. Such as, the rodent repeat database are going to be chosen if "Mouse" is specified in "Organism" area.
In contrast to random primers, oligo (dT) primers are certainly not ideal for degraded samples but are outstanding for constructing cDNA libraries and encouraged to be used with reverse transcriptases (RTs) as Superscript IV RT.
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Let’s get going, but to start with…just a little disclaimer this is click here only academic. Take treatment in reloading given that it more info may be a potentially perilous interest.
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